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KMID : 0043320180410010087
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Archives of Pharmacal Research
2018 Volume.41 No. 1 p.87 ~ p.100
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Proteomic and transcriptomic analysis of lung tissue in OVA-challenged mice
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Lee Yong-Jin
Hwang Yun-Ho
Kim Kwang-Jin
Park Ae-Kyung
Paik Man-Jeong
Kim Seong-Hwan
Lee Su-Ui
Yee Sung-Tae
Son Young-Jin
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Abstract
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Asthma is a long term inflammatory disease of the airway of lungs characterized by variable airflow obstruction and bronchospasm. Asthma is caused by a complex combination of environmental and genetic interactions. In this study, we conducted proteomic analysis of samples derived from control and OVA challenged mice for environmental respiratory disease by using 2-D gel electrophoresis. In addition, we explored the genes associated with the environmental substances that cause respiratory disease and conducted RNA-seq by next-generation sequencing. Proteomic analysis revealed 7 up-regulated (keratin KB40, CRP, HSP27, chaperonin containing TCP-1, TCP-10, keratin, and albumin) and 3 down-regulated proteins (PLC-α, PLA2, and precursor ApoA-1). The expression diversity of many genes was found in the lung tissue of OVA challenged moue by RNA-seq. 146 genes were identified as significantly differentially expressed by OVA treatment, and 118 genes of the 146 differentially expressed genes were up-regulated and 28 genes were downregulated. These genes were related to inflammation, mucin production, and airway remodeling. The results presented herein enable diagnosis and the identification of quantitative markers to monitor the progression of environmental respiratory disease using proteomics and genomic approaches.
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KEYWORD
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Asthma, Ovalbumin (OVA), Environmental respiratory disease, Proteomics, RNA-seq
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